FlyBase Gene Report: Dmel\Ac13E

General Information

Symbol

Dmel\Ac13E

Species

D. melanogaster

Name

Adenylyl cyclase 13E

Annotation Symbol

CG9210

Feature Type

protein_coding_gene

FlyBase ID

FBgn0022710

Gene Model Status

Current

Stock Availability

6 publicly available

Gene Snapshot

Contributions welcome

Other Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

DAC9, Adenylyl cyclase 35C

Key Links

Genomic Location

Cytogenetic map

13C7-13C8

Sequence location

X:15,510,877..15,554,278 [-]

Recombination map

1-50

RefSeq locus

NC_004354 REGION:15510877..15554278

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (5 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables adenylate cyclase activity

inferred from direct assay

(Iourgenko et al., 1997)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables adenylate cyclase activity

inferred from biological aspect of ancestor with PANTHER:PTN002899368

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in adenylate cyclase-activating G protein-coupled receptor signaling pathway

inferred from biological aspect of ancestor with PANTHER:PTN002899368

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in cyclic nucleotide biosynthetic process

inferred from electronic annotation with InterPro:IPR001054, InterPro:IPR018297

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in integral component of membrane

inferred from sequence model

(Iourgenko et al., 1997)

located_in integral component of plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN002899368

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

ADENYLATE CYCLASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

ADENYLATE CYCLASES -: Adenylate cyclases catalyze the synthesis of cAMP from ATP, yielding diphosphate as a by-product. (Adapted from FBrf0238856).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ac13E for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.45

Gene model reviewed during 5.55

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ac13E-RA

FBtr0074073

NM_057951

6589

1690

Ac13E-RB

FBtr0100142

NM_001014745

7170

1690

Ac13E-RC

FBtr0332497

NM_001272646

7565

1703

Additional Transcript Data and Comments

Reported size (kB)

8.9, 7.5, 7.2 (northern blot)

(Iourgenko et al., 1997)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Ac13E-PA

FBpp0073889

186.9

1690

6.74

NP_477299

AAF48468

Ac13E-PB

FBpp0099492

186.9

1690

6.74

NP_001014745

AAX52499

Ac13E-PC

FBpp0304773

188.3

1703

6.81

NP_001259575

AGB95417

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1690 aa isoforms: Ac13E-PA, Ac13E-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

1708 (aa)

(Iourgenko et al., 1997)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ac13E using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Iourgenko et al., 1997)

adult stage

(Iourgenko et al., 1997)

larval stage

(Iourgenko et al., 1997)

Additional Descriptive Data

The 7.5kb Ac13E transcript is abundant in adults.

(Iourgenko et al., 1997)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Ac13E in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ac13E

View exonic expression by tissue for Dmel\Ac13E

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0022710

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG9210-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9210

Flygut - An atlas of the Drosophila adult midgut

FBgn0022710

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ac13E

Transgenic constructs containing regulatory region of Ac13E

Deletions and Duplications ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Ac13E^GD3374

viable, with Scer\GAL4^Mef2.PR

Ac13E^GD3374

viable, with Scer\GAL4^pnr-MD237

Ac13E^GD3374

viable, with Scer\GAL4^tin.CΔ4

Ac13E^GD3374

Other Phenotypes

Allele

visible, with Scer\GAL4^pnr-MD237

Ac13E^GD3374

Phenotype manifest in

Allele

trichogen cell, with Scer\GAL4^pnr-MD237

Ac13E^GD3374

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (22)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\ADCY9

14 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (24)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Adcy9

12 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (21)

Rnor\Adcy9

8 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (14)

Xtro\adcy9

8 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (31)

Drer\adcy9

6 of 15

Yes

Drer\LOC101884360

2 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Drer\si:ch211-132f19.7

1 of 15

Drer\si:dkey-37g12.1

1 of 15

Drer\si:dkey-206f10.1

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (39)

Cele\acy-1

14 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\CYR1

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091900K3 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA21615

Drosophila persimilis

Dper\GL13305

Drosophila willistoni

Dwil\GK20007

Drosophila virilis

Dvir\GJ18814

Drosophila mojavensis

Dmoj\GI15443

Drosophila grimshawi

Dgri\GH24079

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500DG )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17018338

Glossina morsitans

Tsetse fly

Gmor\GMOY004274

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_00187

Mayetiola destructor

Hessian fly

Mdes\Mdes006768

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL017261

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL012294

Anopheles darlingi

American malaria mosquito

Adar\ADAC007341

Anopheles gambiae

Malaria mosquito

Agam\AGAP000090

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ017308

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W01HK )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA001254

Bombyx mori

Silkmoth

Bmor\BGIBMGA001255

Danaus plexippus

Monarch butterfly

Dple\DPOGS211881-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL007706

Apis florea

Little honeybee

Aflo\2416051668

Apis mellifera

Western honey bee

Amel\GB47271

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP17160

Bombus terrestris

Buff-tailed bumblebee

Bter\15212478

Linepithema humile

Argentine ant

Lhum\LH23365

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000f6d

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG011983

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_00106

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_01828

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_09026

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_07814

Pediculus humanus

Human body louse

Phum\PHUM155150

Rhodnius prolixus

Kissing bug

Rpro\RPRC004423

Cimex lectularius

Bed bug

Clec\CLEC010733-PB

Cimex lectularius

Bed bug

Clec\CLEC010733

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_13369

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01FQ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR008293

Ixodes scapularis

Black-legged tick

Isca\ISCW021824

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_302912

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0285 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Adcy9_1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Adcy9

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000007249

Gallus gallus

Domestic chicken

Ggal\ADCY9

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (27)

Gene Symbol

Score

Source

Alignment

4 of 10

Ac3

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

rut

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ADCY9; adenylate cyclase 9

14 of 15

603302

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0022710

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-50

Cytogenetic map

13C7-13C8

Sequence location

X:15,510,877..15,554,278 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

13C7-13C8

Limits computationally determined from genome sequence between P{EP}CG6340^EP1109&P{EP}l(1)G0168^EP491 and P{EP}EP1489

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

13E-13E

(Iourgenko, 1997.5.27)

Experimentally Determined Recombination Data

Location

1-50

(FlyBase, 2016)

1-50.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (6)

Bloomington

27764

w¹¹¹⁸ Mi{ET1}Ac13E^MB08864

84198

y¹ v¹; M{WKO.P2-A1}ZH-86Fb

42040

y¹ w^* Mi{MIC}Ac13E^MI00983

62247

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05254}attP40

VDRC

v110750

P{KK108123}VIE-260B

v11547

w¹¹¹⁸; P{GD3374}v11547

Genomic Clones (20)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (8)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0022710

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0022710

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

32485

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Ac13E CG9210

(Wang, 1999.11)

Source for database merge of

Additional comments

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Biochemical analysis confirms Ac13E encodes a functional enzyme that can be activated by forskolin or G protein.

(Iourgenko et al., 1997)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 48 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32485

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0022710

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9210

Flygut - An atlas of the Drosophila adult midgut

FBgn0022710

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

32485

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC007814

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG9210

modMine - A data warehouse for the modENCODE project

FBgn0022710

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0022710

DRSC - Results frm RNAi screens

FBgn0022710

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0022710

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG9210-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0022710

FlyMine - An integrated database for Drosophila genomics

FBgn0022710

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

AC 13E

(Morrison et al., 2000)

AC13E

(Duvall and Taghert, 2013, Duvall and Taghert, 2012, Ueno and Kidokoro, 2008)

Ac13E

(Meltzer, 2019-, Meltzer et al., 2019, Michalak et al., 2019, Franz et al., 2017, Ashwal-Fluss et al., 2014, Felix et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Guan et al., 2011, Wasbrough et al., 2010, Wang et al., 2004)

Ac35C

CG9210

(Hosono et al., 2015, Keleman et al., 2009.8.5, Perkins et al., 2009, Morrison et al., 2000)

DAC9

(Cann and Levin, 2000, Cann et al., 2000, Iourgenko and Levin, 2000, Cann and Levin, 1998, Iourgenko, 1997.5.27, Iourgenko et al., 1997, Iourgenko et al., 1997)

ac13e

(Naganos et al., 2016)

Name Synonyms

Adenyly cyclase 35C

(Guan et al., 2011)

Adenylyl cyclase 13E

Adenylyl cyclase 35C

(Felix et al., 2012, Iourgenko et al., 1997)

adenylyl cyclase

(Yovell et al., 1992)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (54)

The following external sites may use different assemblies or annotations than FlyBase.

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