Subject: Re: FlyBase Query (cy1797)
...
> GFP-Arm
> =======
> You describe GFP-Arm construct, which you got from C. Alexandre. Do
> you have any more molecular details on this construct? Is it a 'true'
> mini-gene, i.e. constructed from genomic DNA. Is (as the name suggests)
> the GFP inserted at the N terminus? What marker does the transposon
> carry?
just to recap:
The GFP cDNA has been fused to the N terminus of Arm cDNA. The
mini-gene arm pro-GFPARM has been created by cloning the fusion
downstream of 2 kb arm promoter. The mini-gene has been inserted in
pCasper4 so it's with white as a marker.
\--
Cyrille ALEXANDRE
Division of Mammalian Development, Room 108
National Institute for Medical Research (N.I.M.R)
The Ridgeway, Mill Hill
LONDON NW7 1AA
UK