Subject: Fwd: FlyBase Query (cy1763) (fwd)
Hi Chihiro,
I answer to your questions...
Df(3L)XZB970 was reported in Rebay et al, Genetics 154(2), 695-712,
2000. It complements trachealess, and fails to complement nurf301 and
CG7036.
Df(3L)rH321 is originally from the Berkeley P-element insertion
collection, known as l(3)rH321. Matt Voas inverse PCRed off the 5' and
3' ends of this insertion and realized that they lie about 50kb apart.
The proximal end is to the right of the nurf301 coding region and the
distal end is to the right of the CG7036 coding region. He confirmed
by PCR that this region is deleted rather than having two insertions
that would confound the inverse PCR results. Genetically, Df(3L)rH321
fails to complement nurf301 but complements CG7036.
Df(3l)3643 is an excision line derived from EP(3)3643. It removes
nurf301 entirely. I mapped the proximal to within 2 kb of the nurf301
ATG. It leaves CG7020 intact. Distally, the deficiency removes at
least to CG17142, however this breakpoint has not been precisely
mapped.
I hope this helps.
Sincerely,
Paul.
> \---------- Forwarded message \----------
> Date: Tue, 11 Feb 2003  18:21:49  \+0000
> Subject: FlyBase Query (cy1763)
>
> Dear Dr Wu,
>
> I am currently curating your paper for FlyBase:
>
> Badenhorst et al., Genes Dev. 2002 16(24), 3186--3198
>
> I have a few question I was hoping you could answer for me.
>
> Df(3L)XZB970
> ============
> We don't have a Deficiency of this name in our records. Has it been
> published before? If so under what name?
>
> Df(3L)rH321
> ===========
> We don't have a Deficiency of this name in our records. Has it been
> published before? If so under what name? What relationship does it
> have to the insertion P{PZ}l(3)06240rH321, Was this insertion used to
> make this line by imprecise excision?
>
> Df(3L)3643
> ==========
> You make this Deficiency from P{EP}EP3643. Do you any information
> about the breakpoints in this Df? Are either of them within the
> nurf301 gene?
>
> Any new information you give us will be curated as a personal
> communication form you to FlyBase, if thats fine with you.
>
> Best wishes,
>
> Chihiro
>
> \----------------------------------------------------------------------
> Chihiro Yamada.
>
> FlyBase (Cambridge)
> Department of Genetics,
> \----------------------------------------------------------------------
Paul Badenhorst
NCI Laboratory of Molecular Cell Biology
Bldg 37 Rm 6066
37 Convent Drive
Bethesda MD 20892-4255
USA