Subject: Components of stocks from the Michelson lab
Personal communication from: Bloomington Drosophila Stock Center
Subject: Components of stocks from the Michelson lab
These insertions are described, named and sequence-mapped in FBrf0152364,
Halfon et al., genesis 2002. The cytology below is inferred from the
insertion site given in the publication.
Enhanced GFP insertions
P{UAS-2xEGFP}AH2 28F3-5
P{UAS-2xEGFP}AH3 70D
Enhanced YFP insertions
P{UAS-2xEYFP}AX 10B
P{UAS-2xEYFP}AH2 58D8
P{UAS-2xEYFP}AH3 68C
These insertions are described but not named in FBrf0152364, Halfon et al.,
genesis 2002. They were generated by hopping an unnamed 2nd chromosome
insertion of P{GAL4-twi.G} described in FBrf0059139, Greig and Akam, Nature
1993. They map to the chromosome indicated. The insertion identifiers are
those used in the Bloomington stock list.
Twist-GAL4 insertions
P{GAL4-twi.G}2.2 2-
P{GAL4-twi.G}2.3 3-
These balancers expressing enhanced GFP from embryonic stage 8 through adult
are described in FBrf0152364, Halfon et al., genesis 2002. They were
generated by hopping P{UAS-2xEGFP}AH2 into CyO and TM3 chromosomes. The
symbol used by the donor and the balancer short genotype used in the
Bloomington stock list are shown.
CTG CyO, P{w+mC=GAL4-twi.G}2.2, P{UAS-2xEGFP}AH2.2
TTG TM3, P{w+mC=GAL4-twi.G}2.3, P{UAS-2xEGFP}AH2.3, Sb1 Ser1
An additional balancer was mentioned by not further described in FBrf0152364,
Halfon et al., genesis 2002. It carries an otherwise undescribed insertion of
P{UAS-2xEGFP} on chromosome 1 that we have called AX, in keeping with other
names in this set. The identify of the P{GAL4-twi.G} insertion is our best
guess.
FM7c, P{w+mC=GAL4-twi.G}108.4, P{UAS-2xEGFP}AX