Subject: Revised cytological breakpoints for Df(2L)spd and Df(2L)XE-2750 Revised cytological breakpoints for Df(2L)spd and Df(2L)XE-2750 Df(2L)XE-2750 28A4 present, 28B1,2 doublet absent, 28C1 absent, 28D1,2 doublet present. It's also likely that 28C7,8 present, but conservative interpretation is 28A5-B1;28C1-9. Df(2L)spd Though reported in literature as a simple deletion, it is really a deficient inversion. The breakpoints are 26A6-B1;27E1-3;28B1-3. Inversion breakpoint between 26A6 and 26B1. 27E1,2 doublet may be partially deleted. 27E3 definitely deleted. Deletion breakpoint between 26B1,2 and B3. 28C1 present. Therefore, cytology is 27E1-3;28B1-3;26A6-B1 with the deletion between the first two breakpoints.