Subject: Fwd: FlyBase Curation Query
A. Spradling provided two lacZ enhancer trap insertions in esg,
esg05729 and esg05730. The insertion sites for both enhancer traps
were mapped to the same location, 410bp upstream of the esg
transcriptional start site. We used these enhancer trap insertions
as targets for a P-element mediated replacement with a P{GawB} line
(Sepp and Auld, 1999) to obtain GAL4 expressing enhancer trap lines
in esg. We obtained 4 GAL4 insertions in the esg locus but the
original lacZ insertion remained in each case.
Those 4 GAL4 insertions are cleverly named A, B, C and D. All four
recreate the esg expression pattern in embryos and larvae. I've used
B since I had some initial problems with A.
Audrey Christiansen
>>Subject: FlyBase Curation Query
>>
>>Dear Bruce,
>>
>>Do you have information you could send me on 'esgGAL4.B, isolated
>>by A. Christiansen' as mentioined in your paper 'Keisman et al.,
>>2001, Dev. Cell 1(2): 215--225'. The minimum I need to know to
>>create a record for it is whether it is an enhancer trap or a
>>promoter/enhancer fusion? Any molecular details you can provide
>>could also be included in a curation record and an archived
>>presonal communication.