Isolation and characterization of Df(3L)BSC35
Rachel Andrade and Kevin Cook
Bloomington Drosophila Stock Center, Indiana University
Df(3L)BSC35 was isolated as a P transposase-induced male recombination event involving P{EP}smgEP3556 and P{lacW}l(3)j2B9j2B9. The deletion was isolated as a rhove-1-e1 recombinant chromosome from the cross rhove-1 pp e1 females x CyO, H{PDelta2-3}HoP2.1/+; rhove-1 P{EP}smgEP3556/P{lacW}l(3)j2B9j2B9 e1 males. The mini-white marker from at least one insertion was retained on the deletion chromosome. Polytene chromosome squashes showed the breakpoints 66F1-2;67B2-3 (the distal breakpoint fell within the 66F1,2 doublet band, leaving a portion of distal 66F1,2 undeleted). Df(3L)BSC35 failed to complement Rdl1, P{PZ}bol1 and P{lacW}aayS042314.