Dear Chihiro, .. UAS-EGFPF =========== The membrane-targeted reporter UAS-EGFPF used in our study was generated (by us) by N-terminal insertion of 6 copies of the c-myc epitope tag into the previously generated UAS-EGFPF (Finley et al. 1998), a C-terminal insertion into EGFP of the carboxy-terminal 20 amino acids of c-Ha-Ras containing membrane-targeting farnesylation and palmitoylation sequences (Jiang and Hunter, 1998). Finley et al. construct, denoted UAS-EGFPF: UAS::EGFP::CAAX Allan et al. construct, denoted UAS-EGFPF: UAS::6xMyc::EGFP::CAAX Finley, et al. (1998). Neuron 21, 1363-1374. Jiang, W., and Hunter, T. (1998). Biotechniques 24, 348-354. We should have called it UAS-myc-EGFPF. This was a mistake. Hope that this is helpful, best wishes, \-- Stefan Thor Ph.D. Assistant Professor of Neurobiology Department of Neurobiology, GB 504 Harvard Medical School 220 Longwood Avenue Boston, MA 02115
