Subject: FlyBase query
Dear Dr. Dahmann,
I am curating a paper for FlyBase:
Rintelen et al., 2003, Development 130(15): 3479--3490
in which the authors use 2 engrailed-GAL4 lines from you:
en-GAL454 and en-GAL433 (<up></up> = superscript)
I wrote to the authors about these lines as I was not sure whether or
not we already have them in FlyBase, and Knud Nairz replied that 'Those
lines have been generated from the lacZ enhancer trap line by
gene-conversion. Possibly they have not been put into FlyBase.'
so I am still unsure whether or not we have a record of these lines in
FlyBase, although I suspect that they are new.
I was hoping you could tell me the details of how you made the two
lines so that I can get this information into the database (I would
record this information as a personal communication from you).
Since they were made by gene conversion, I would be grateful if you
could tell me:
a. the starting lacZ enhancer trap line, with its identifier number,
e.g. 24B, A101.
b. what is the GAL4 vector that was used for the gene conversion \- was
it the GawB element ?
c. are the '54' and '33' lines just two different cases of gene
conversion that were recovered,
thanks,
Gillian
\--------------------------------------------------------------
Gillian Millburn.
FlyBase (Cambridge),
\--------------------------------------------------------------
>
Subject: Re: FlyBase query
Dear Gillian,
The starting lacZ enhancer trap line for en-GAL454 and en-GAL433 (<up></up> =
superscript) was enryXho25 (Hama et al. (1990) Genes and Development
 4:1079-1093 )).
The GAL4 vector that was used for the gene conversion is unknown.
The lines '54' and '33' are two independent cases of gene conversion.
Regards,
Christian Dahmann
> Dear Dr. Dahmann,
>
> I am curating a paper for FlyBase:
>
> Rintelen et al., 2003, Development 130(15): 3479--3490
>
> in which the authors use 2 engrailed-GAL4 lines from you:
>
> en-GAL454 and en-GAL433 (<up></up> = superscript)
>
> I wrote to the authors about these lines as I was not sure whether or
> not we already have them in FlyBase, and Knud Nairz replied that 'Those
> lines have been generated from the lacZ enhancer trap line by
> gene-conversion. Possibly they have not been put into FlyBase.'
>
> so I am still unsure whether or not we have a record of these lines in
> FlyBase, although I suspect that they are new.
>
> I was hoping you could tell me the details of how you made the two
> lines so that I can get this information into the database (I would
> record this information as a personal communication from you).
>
> Since they were made by gene conversion, I would be grateful if you
> could tell me:
>
> a. the starting lacZ enhancer trap line, with its identifier number,
> e.g. 24B, A101.
>
> b. what is the GAL4 vector that was used for the gene conversion \- was
> it the GawB element ?
>
> c. are the '54' and '33' lines just two different cases of gene
> conversion that were recovered,
>
> thanks,
>
> Gillian
>
> \--------------------------------------------------------------
> Gillian Millburn.
>
> FlyBase (Cambridge),
>
> \--------------------------------------------------------------
>