Subject: Nmdar1 constructs and insertions
The following information was provided by Annette Parks of Exelixis, Inc.
In P{UAS-Nmdar1.Exel}, wild type Nmdar1 (FBgn0010399) coding sequences were
cloned into P{Express-UAS}. May be missing amino acids 571-573, 833-855
and 965-969.
P{UAS-Nmdar1.Exel}1 is a homozygous and hemizygous viable and fertile, X
chromosome insertion.
P{UAS-Nmdar1.Exel}2 is a homozygous viable and fertile, second chromosome
insertion.
In P{GMR-Nmdar1.Exel},wild type Nmdar1 coding sequences were cloned into
P{Express-glass}. May be missing amino acids 571-573, 833-855 and 965-969.
P{GMR-Nmdar1.Exel}1 is an X chromosome insertion.
P{GMR-Nmdar1.Exel}3 is a homozygous viable and fertile, third chromosome
insertion.
In P{UAS-Nmdar1.lurcher}, Nmdar1 sequences carrying the mouse lurcher
mutation were cloned into P{Express-UAS}. The lurcher mutation changes a
conserved YTANLAAF to YTANLATF.
P{UAS-Nmdar1.lurcher}2 is a homozygous viable and fertile, second
chromosome insertion.
P{UAS-Nmdar1.lurcher}3 is a homozygous viable and fertile, third chromosome
insertion.
In P{GMR-Nmdar1.lurcher}, Nmdar1 sequences carrying the mouse lurcher
mutation were cloned into P{Express-glass}. The lurcher mutation changes a
conserved YTANLAAF to YTANLATF.
P{GMR-Nmdar1.lurcher}1 is a homozygous and hemizygous viable and fertile, X
chromosome insertion.
P{GMR-Nmdar1.lurcher}2 is a homozygous viable and fertile, second
chromosome insertion.
P{GMR-Nmdar1.lurcher}3 is a homozygous viable and fertile, third chromosome
insertion.