Subject: polo constructs and insertions
The following information was provided by Annette Parks of Exelixis, Inc.
In P{UAS-polo.T182D}, the polo (FBgn0003124) coding sequence with a T182D
change was cloned into P{Express-UAS}. The encoded mutant polo protein
should be constitutively active and equivalent to the human mutant T210D
protein.
P{UAS-polo.T182D}3 is a homozygous viable and fertile, third chromosome
insertion.
In P{GMR-polo.T182D}, the polo coding sequence with a T182D change was
cloned into P{Express-glass}.
P{GMR-polo.T182D}2 is a homozygous viable and fertile, second chromosome
insertion.