Subject: arm constructs and insertions
The following information was provided by Annette Parks of Exelixis, Inc.
In P{UAS-arm.Exel}, wild type arm (FBgn0000117) was cloned into
P{Express-UAS}. The arm sequence was PCR amplified using forward primer
GCGCGCGCGGCCGCGCAGATAAATTTACTT and reverse primer
GCGCGCTCTAGAGGAATTCGTGTGTTTTAT, cloned into TOPO TA and shuttled into
P{Express-UAS}.
P{UAS-arm.Exel}1 is an X chromosome insertion.
P{UAS-arm.Exel}2 is a homozygous viable and fertile, second chromosome
insertion.
P{UAS-arm.Exel}3 is a third chromosome insertion.
In P{GMR-arm.Exel}, wild type arm was cloned into P{Express-glass}. The arm
sequence was PCR amplified using forward primer
GCGCGCGCGGCCGCGCAGATAAATTTACTT and reverse primer
GCGCGCTCTAGAGGAATTCGTGTGTTTTAT, cloned into TOPO TA and shuttled into
P{Express-glass}.
P{GMR-arm.Exel}3 is a homozygous viable and fertile, third chromosome
insertion.
In P{vgMQ-arm.Ndel}, the arm gene deleted for the first 128 amino acids was
cloned into P{Express-vgMQ}. The encoded mutant protein is constitutively
active.
P{vgMQ-arm.Ndel}1 is a homozygous and hemizygous viable and fertile, X
chromosome insertion.
P{vgMQ-arm.Ndel}2 is a second chromosome insertion.
P{vgMQ-arm.Ndel}3 is a third chromosome insertion.
In P{GMR-arm.S56F}, arm sequences encoding a S56F variant protein were
cloned into P{Express-glass}. The mutant protein is constitutively
active. The S56F change mimics the S45F change in humans.
P{GMR-arm.S56F}1 is a homozygous and hemizygous viable and fertile, X
chromosome insertion.
P{GMR-arm.S56F}2 is a second chromosome insertion
P{GMR-arm.S56F}3 is a third chromosome insertion.
In P{GMR-arm.T52A}, arm sequences encoding a T52A variant protein were
cloned into P{Express-glass}. The mutant protein is constitutively
active. The T52A change mimics the T41A change in humans.
P{GMR-arm.T52A}2 is a homozygous viable and fertile, second chromosome
insertion.