Subject: wfs1 constructs and insertions
The following information was provided by Annette Parks of Exelixis, Inc.
CG4917 (FBgn0039003) is the fly homolog of the human Wolfram syndrome 1
(WFS1) gene. The human protein is also called wolframin in some references.
In P{GMR-wfs1.Exel}, full length wfs1 was cloned into
P{Express-glass}. The sequence of wfs1 in this construct and the two
others below is not identical to other sequenced clones. Its sequence is:
MATWTQNEPTGVTKRRRWNLEDRASLNKLKHHIAEEGCPQMQYDLAKELLDNSIEPNLAKGNQSQKAVNWLVSAAHNGH
EDAVKLLRQCYNDGSGITAENTDEVRRCLAMTPGERAARKAARELFACLSNGNEHITPKQLERKMRRIYNLQRKRRRRD
DDRSSSSSEGEQEPECEPLEDVPTIDLANVERRRLITEAHLVSAASNYSAGQMPSVNDALTLSVPDPRSLDHVPCFYRM
IFHPLIFFTLFYHRLLNLIVSIPNVIPLSVRCSVLVAISWWSSRHMLPLVSYYLSLGIMIWATCKMLKTKQQFVDFRIW
SGLFLSYGDQNIEADIAEHRFLRNNMKPYLYFFCAFICNLIVYPLVTDAWLPHSELTIISGALTFITMCVSMYASSHQL
PDWLVIVSFAVNVLAKYPYEMDEVVSTRWRFLDLRVPTFSSFVIGNGIEFCLNCRTALYLFIPVLLIMMAKRSRWHGVY
TFLIPHCVTLSWLQVCIATSQSSTVFGVMRAALGLAGIVLFLPLFGIVALLVPVFVAIDSLGLASEQLRWGSTALACGL
VVVLSCILALNRATQKYITMLQLITAITTACLLVLPYMTSSFKDTPRFNAMPRAGLHSLSETNTLPWDRFHALCAQPVH
EQPNKIKAQLRCSLLNGMPVIWEGSVTKVEISRVSNFLEDTIANYLPVWLGRMLRCLHGENISQHFKCDPKLDAQCEEW
RSVFKTFNAQSGSCTLQRWNRYEYELLVKVGTKRSGRLLGRSTTTDVILRAHHDFGNFTRLLSEGDVVLFYGILHNSRL
LADNVQVKLKTIECVECRSRDLGTASIERVVAASPMDARLQDLMRGIKYLLNALLNPLITFK
P{GMR-wfs1.Exel}1 is a homozygous and hemizygous viable and fertile, X
chromosome insertion.
P{GMR-wfs1.Exel}2 is a homozygous viable and fertile, second chromosome
insertion.
P{GMR-wfs1.Exel}3 is a homozygous viable and fertile, third chromosome
insertion.
In P{UAS-wfs1.Exel}, full length wfs1 was cloned into P{Express-UAS}.
P{UAS-wfs1.Exel}2 is a homozygous viable and fertile, second chromosome
insertion.
P{UAS-wfs1.Exel}3 is a homozygous viable and fertile, third chromosome
insertion.
In P{sev-wfs1.Exel}, full length wfs1 was cloned into either
P{Express-sev1x} or P{Express-sev3x}.
P{sev-wfs1.Exel}1 is a homozygous and hemizygous viable and fertile, X
chromosome insertion.
P{sev-wfs1.Exel}2 is a homozygous viable and fertile, second chromosome
insertion.
P{sev-wfs1.Exel}3 is a homozygous viable and fertile, third chromosome
insertion.