Subject: P{UAS-Pten.dsRNA.Exel} construct and insertions
The following information was provided by Annette Parks of Exelixis, Inc
(6/04).
P{UAS-Pten.dsRNA.Exel} is a transgene construct which generates dsRNA
transcripts for RNA interference of the Pten gene. Nucleotides 295 through
819 from Pten sequence AF201905 were cloned in the forward and reverse
directions into pJM1084 (the hairpin vector described in Reichhart et al.
2002, genesis  34:160-164 ; FBrf0152370). The cloned sequences differed from
AF201905 by an A to C change at nucleotide 423.
P{UAS-Pten.dsRNA.Exel}1 is a homozygous and hemizygous viable and fertile,
X chromosome insertion.
P{UAS-Pten.dsRNA.Exel}2 is a homozygous viable and fertile, second
chromosome insertion.
P{UAS-Pten.dsRNA.Exel}3 is a homozygous viable and fertile, third
chromosome insertion.