Subject: Unpublished Data Submission to FlyBase
Dear Sir,
We would like to submit the data we have obtained for A1-2-29
enhancer-trap line to FlyBase, since we are not planning to publish
the data.
We rescued both 5' and 3' flanking genomic sequence of the pLacW
insertion by plasmid rescue method and the details are shown below.
Enhancer-trap line: A1-2-29
Reference: Hartenstein and Jan 1992, FBrf0057603
Insertion was found to be upstream of the gene Rapgap1 FBgn0014015
3' rescued sequence \+/- (starting at 2L: 7522842)
CATGGGCAGAGGCAGCAAACAAC
ATTGGATATTTTTCAGTTGCCGTTTCTCCGTTGCCGAAAGCAGACGTTTGAGCCGGTTTGAATTC
5' rescued sequence \+/+ (starting at 2L: 7522829)
TGCCTCTGCCGATGCGTCTGTCG
CTCTGCTCTGCCAACGCACAGTG
GGCCAGACACAACAAGACTCGAT
GTCTAAGAAAAGCGCATTTTGCG
TACGTATGTTATCCATATGGGGA
TTTGTAAAAATTTCTGTTATTTA
TAACATACATTATTCTATTATATACAAAATGGTTTTTTGAGAAATTTA
Reference for Rapgap1:
Biological characterization of Drosophila Rapgap1, a GTPase
activating protein for Rap1.
Chen, F., et al.
Proc. Natl. Acad. Sci. USA
Vol. 94, pp. 12485-12490 1997
If you use this Information, please quote pers. comm. from: Kanagawa S. and
Moore A.W.
Regards
Adrian Moore
Dr Adrian Moore,
Unit Leader,
Molecular Neuropathology Group,
RIKEN Brain Science Institute,
2-1 Hirosawa, Wako City, Saitama
351-0198 JAPAN