Subject: Re: <up>Fwd: FlyBase Query (cy2646)</up>
Dear Chihiro,
The Vav gene that we used to construct the Vav-GFP described
in Fig 5 c,f is indeed VAV1 ( GDB:127112 ). It was cloned into the
pUAST vector, and expressed as clones under the control of a hsFlp;
abxubs>FRT>Gal4 driver.
Hope this helps.
regards,
Frank Hsiung
>Dear Dr Kornberg,
>
>I am currently curating your paper for FlyBase:
>
>Hsiung et al. 2005, Nature 437(7058) 560--563.
>
>I have a quick question I was hoping you could answer for me.
>
>Vav-GFP
>=======
>In Figure 5 c,f you show Vav-GFP labelled clones.
>
>I believe the Vav gene you are talking about is called VAV1 by GDB
>( GDB:127112 ). Can you confirm this? Secondly, could you givw me some
>molecular details about this construct? e.g. What promoter drives
>expression of the Vav-GFP?
>
>Best wishes,
>
>Chihiro