Subject: Re: FlyBase Curation query
David,
There is one 'wild-type' UAS-runt construct that we made that is
distributed. The first publication to mention this is the Li and
Gergen paper, although thework in the Tracey et al paper really
provides the detailed characterization of this construct in the
context of using the maternal GAL4 (NGT) constructs that were also
developed in the lab. Hope this clears things up.
Peter Gergen
>Dear Peter,
>
>Do you have time to help me clear up a question I have about constructs
>generated in your lab?
>
>We currently have 2 separate records for UAS-runt constructs made in
>your lab.
>These come from our curation of the papers:
>
>FBrf0110190 == Li and Gergen, 1999, Development 126(15): 3313--3322
>&
>FBrf0123220 == Tracey et al., 2000, Genetics 154(1): 273--284
>
>It struck me as unlikely that you would have made the same construct twice in
>your lab, especially within such a short span of time. Are they the same
>construct, or is there some distinction?