The following information accompanied stocks donated to the Bloomington Stock Center by Marc Bourouis, Université de Nice (3/05).
P{UAS-sgg.KK83-84MI} expresses a kinase dead form of sgg protein, presumably due to a misfolded kinase. It does not have dominant negative activity on wingless signaling.
P{UAS-sgg.KK83-84RK} expresses a near kinase dead form of sgg protein, which has dominant negative activity on wingless signaling.
P{UAS-sgg.KK83-84MI} and P{UAS-sgg.KK83-84RK} were produced and assayed as in Bourouis, 2002, 'Targeted increase in Shaggy activity levels blocks wingless signaling', Genesis 34: 99-102 (FBrf0152388).
P{UAS-sgg.KK83-84MI}2.1 and P{UAS-sgg.KK83-84RK}6 are homozygous viable and fertile, third chromosome insertions.