Subject: Re: Helping FlyBase: 'UAS-Pvf1'
Dear Raquel:
I transcribe below the answer of Dra Rorth, I expect with this we can solve
the problem.
Sincerely Ana Macías
Dear Ana
I don't think we have published the UAS-PVF1. The vector is UASp2 (UASp
with new polylinker) and the insert from the pvf1 cDNA LD30334. You can
cite me and this unpublished data for flybase
Pernille
\----- Original Message \-----
Sent: Monday, May 09, 2005  12:43  PM
Subject: Helping FlyBase: 'UAS-Pvf1'
> Dear Ana,
>
> I am now working on your paper \- getting the data tracked in FlyBase \-
>
> FBrf0184038 == Macias et al., 2004, Int. J. Dev. Biol. 48(10): 1087--1094
> 'PVF1/PVR signaling and apoptosis promotes the rotation and dorsal closure
of the Drosophila male terminalia.'
>
> and have a question about one of your constructs. You use 'UAS-Pvf1'
> (e.g. in 'Pvf1 gain of function' section, p1090) and reference
> FBrf0139607 == Duchek et al., 2001, Cell 107(1): 17--26
> in your Materials and Methods.
>
> However the FBrf0139607 paper does not include a 'UAS-Pvf1', unless you
> mean Pvf1EP1624 or Pvf1EPg11235. We do have two 'UAS-Pvf1'
> constructs known to FlyBase:
>
> P{UAS-Pvf1.R}
> described in
> FBrf0174570 == Rosin et al., 2004, Development 131(9): 1939--1948
>
> and
> P{UAS-Pvf1.M}
> described in
> FBrf0160786 == McDonald et al., 2003, Development 130(15): 3469--3478
>
> Might one of these be the one you used?
>
> Thanks for help,
>
> With best regards,
>
> Rachel.