Subject: Re: Question about AmaR1 allele Hi Beverley: The deletion starts with the first base of the P114 codon and ends with the second base of the A123 codon. So these 29 bases are deleted: CCGTACGAGTGTCAGGTGCTTGTTTCGGC Eric On May 20, 2005, at 2:34 PM, Beverley Matthews wrote: > Dear Dr. Liebl, I am a curator for FlyBase and am in the process of curating the molecular nature of the Ama mutations that you reported in a 2003 Development paper, to appear in the genome maps in FlyBase. I have a question about the AmaR1 deletion. From your description, in addition to the missense mutation, there is a 29 base deletion beginning in codon P114 (CCG), resulting in a frameshift. Can you tell me the exact position of the deletion so I can map the mutation accurately in FlyBase? wt 114 MetGlyProTyrGluCysGlnValLeuValSerAlaThrGluLys ATGGGTCCGTACGAGTGTCAGGTGCTTGTTTCGGCCACGGAGAAG Thanks in advance for any help you can offer. Your response will be saved as a Personal Communication to FlyBase and used as a reference for the mapping of the AmaR1 mutation (in addition to your Development paper). Sincerely, Beverley Matthews FlyBase-Harvard