The following information accompanied stocks donated to the Bloomington Stock Center by Kevin K. Hill and J. Michael Bishop, University of California at San Francisco (11/05).
P{lacW}Fps85DE134 is an insertion at coordinate 156723 of AE003682.4, 25 nucleotides upstream of the Fps85D 5' untranslated region.
Df(3R)PX35 is a P transposase-induced deletion derivative of P{lacW}Fps85DE134, which deletes the region from coordinate 156723 (3' Inverted Terminal Repeat of P{lacW}) to 115311 of AE003682.4.
The breakpoint sequence is
3' ITR acgacactca gaatactatt
cctttcactc gcacttattg caagcatacg ttaagtggat gtctcttgcc gacgggacca
ccttatgtta tttcatcatg//deletion//ctttggagct cggggcgaga atcaaaatgg
gcacacgaag ctctgaatgc gtgagcggat gtgtgcatgt gtgcgtgcgc ctgtgtgcaa
taaagagagc.
Df(3R)PX35 removes the genes Fps85D, CG8129, CG8125 and CG16751 and partially removes CG16750.
Fps85DX21 is an X-ray-induced deletion derivative of P{lacW}Fps85DE134, which deletes coordinates 156723 (nucleotide 4807 of P{lacW}) to 147097 of AE003682.4.
The breakpoint sequence is
gggtggtgat tggttttggg tgggtaagcag ggggaaagtg tgaaaaatcc cggcaatggg
ccaagaggat caggagctat taattcgcgg aggcagcaaa//deletion//ggacgcctac
aaagcataaa agtcaccgag gaaaccacgc agtgtcctgc gtccttctgg ctgaaattct
aataaaaccg gcaaatgtca acagggctac.
Exons 1-3 of Fps85D are absent, destroying expression of the p100 and p92 Fps85D isoforms. The p56 and p45 Fps85D isoforms retain expression as determined by RT-PCR.  The lacZ sequence of P{lacW} is retained.
P{lacW}Fps85DX42 was derived from P{lacW}Fps85DE134 by X-ray mutagenesis.  An H.M.S. Beagle element inserted at coordinate 133346 of AE003682.4.  Amino-terminal domains of each Fps85D isoform are expressed separately from the carboxy-terminal SH2 and Kinase domains, i.e. RNA transcripts can be detected carrying exons 1-7 or exons 8-13, but never containing both exons 7 and 8.  The mini-white marker on P{lacW} shows variegated expression.
P{UAS-Fps85D.p45} expresses the p45 isoform of Fps85D under UAS control.
P{UAS-Fps85D.p45}2 is a homozygous viable and fertile, second chromosome insertion.
P{UAS-Fps85D.p92} expresses the p92 isoform of Fps85D under UAS control.
P{UAS-Fps85D.p92}2 is a homozygous viable and fertile, second chromosome insertion.
P{UAS-Fps85D.p100} expresses the p100 isoform of Fps85D under UAS control.
P{UAS-Fps85D.p100}2 is a homozygous viable and fertile, second chromosome insertion.
P{UAS-Fps85D.p92.KM} expresses a kinase-defective version of the p92 isoform of Fps85D under UAS control.
P{UAS-Fps85D.p92.KM}2 is a homozygous viable and fertile, second chromosome insertion.
P{UAS-Fps85D.p92.myr} expresses the p92 isoform of Fps85D with the Src myristoylation sequence (MGSSKSRPKDPSQRLE) at the N-terminus under UAS control.
P{UAS-Fps85D.p92.myr}2 is a homozygous viable and fertile, second chromosome insertion.
P{UAS-Dfer.dsRNA} expresses a double-stranded RNAi construct containing nucleotides 1520-2655 of the p92 Fps85D cDNA encoding the SH2 and Kinase domains.
P{UAS-Fps85D.dsRNA}SK-2 is a homozygous viable and fertile, second chromosome insertion.
P{UAS-Fps85D.dsRNA}SK-3 is a homozygous viable and fertile, third chromosome insertion.
The recessive lethality of the P{hsneo}l(3)neo541 chromosome (currently FBti0005053) may be separated from the P{hsneo} insertion by recombination.  The P{hsneo} insertion lies in or near
Cortactin.  This insertion should be renamed P{hsneo}Cortactinneo54.  The recessive lethality remains unmapped.
Cortactin6A2 is a P transposase-induced deletion derivative of P{hsneo}Cortactinneo54.  Most of the P{hsneo} element is removed with fragments of the 3' Inverted Terminal Repeat remaining (deletion coordinates: 86897 to 84203 of AE003733.3). 94% of the Cortactin protein coding region is absent. The breakpoint sequence is 3' ITR
tttcttgctt tcccatttta cgtaggaatg agaagctgaa tttttcgaat atctctatcc
cccagtgccc tgcttttaa tttagtaatt caatcaactt//deletion//caggcggcgg
atgacgacga gatatccttt gatccagatg acgtgatcac ccacatcgag aagatcgacg
atgggtggtg gaggggattg tgcaagaacc.  Cortactin6A2 is homozygous viable.
P{UAS-Cortactin.HA3} expresses wild type Cortactin epitope-tagged with 3X Haemagluttinin (HA3) at the carboxy terminus under UAS control.
P{UAS-Cortactin.HA3}2 is a homozygous viable and fertile, second chromosome insertion.