Subject: flies are on the way \- descriptions
Dear Rachel and Kevin,
I have just FedEx'ed 6 fly lines to Kevin \- 2 bw; 1 f; and 3 kar.
Attached is a file with their descriptions. Also, I sent the remaining 2
bw lines to Troy Zars.
Please let me know if something goes wrong.
Best, Alex
February 14, 2006
Submission of six strains to the fly collection
All the mutant alleles were produced by EMS.
EMS treatment was done by David Houle (Florida State Univ.), isolation was
done by Alex Kondrashov and Tim Kondrashov, and sequencing was done by Matt
Dean (Univ. of Arizona).
I) Locus bw (brown)
I.1. Allele bw16 (our internal name was bw6, but it is already taken)
GCG > GTG (Ala > Val) missense substitution in codon 78:
CTGGCGGCGATTTCG > CTGGCGGTGATTTCG
I.2. Allele bw19 (our internal name was bw9)
CAG > TAG (Gln > TERM) nonsense substitution in codon 102:
CGGCATCAGATGACG > CGGCATTAGATGACG
II) Locus f (forked)
II.1. Allele f15 (our internal name was f5, but it is already taken)
CAG > TAG (Gln > TERM) nonsense substitution in codon 206 of isoform C:
GTCCAGGATCAAGGT > GTCTAGGATCAAGGT
III) Locus kar (karmoisin)
III.1. Allele kar22 (our internal name was kar2, but it is already taken)
GGC > GAC (Gly > Asp) missense substitution in codon 92:
TGCAACGGCATCATC > TGCAACGACATCATC
III.2. Allele kar23 (our internal name was kar3, but it is already taken)
CCGGTCATGTGTATCGGCATCACC > CCGGTCATCACC (LPVMCIGITS > LPVITS) inframe deletion
of 12 nucleotides (codons 338-341)
III.3.  Allele kar20
TGT > TAT (Cys > Tyr) missense substitution in codon 415:
GAGATCTGTGGCCCC > GAGATCTATGGCCCC