Isolation and characterization of Df(2L)BSC142
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC142 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG7191f03241 and P{XP}CG7356d01144. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}CG7191f03241/P{XP}CG7356d01144 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC142 from the segment of PBac{WH}CG7191f03241 to the left of its FRT site and the segment of P{XP}CG7356d01144 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC142 predicted from the transposable element insertions sites using release 4 coordinates are 28C3;28D3. Df(2L)BSC142 failed to complement mtsXE-2258.