Isolation and characterization of Df(2R)BSC158
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC158 was isolated as a FLP recombinase-induced recombination event involving P{XP}CG12744d01217 and PBac{WH}Pka-R2f07569.  The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}CG12744d01217/PBac{WH}Pka-R2f07569 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females.  These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003).  This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002).  The recombination event generated the genetic element P+PBac{XP5.WH5}BSC158 from the segment of P{XP}CG12744d01217 to the left of its FRT site and the segment of PBac{WH}Pka-R2f07569 to the right of its FRT site.  Its presence was verified using the PCR methods and primers described in Parks et al.  The cytological breakpoints of Df(2R)BSC158 predicted from the transposable element insertions sites using Release 4 coordinates are 46C1;46D1-4.  It failed to complement Adam5-3 and Mef2X1.