Isolation and characterization of Df(3L)BSC157
Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC157 was isolated as a FLP recombinase-induced recombination event involving P{XP}d06654  and PBac{RB}CG6765e00295.  The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6B, Tb1 females crossed to w1118Phs-FLP; P{XP}d06654/PBac{RB}CG6765e00295 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al, Nature Genetics 36: 283-287, 2004; FBrf0175002).  The recombination event generated the genetic element P+PBac{XP5.RB3}BSC157 from the segment of P{XP}d06654 to the left of its FRT site and the segment of PBac{RB}CG6765e00295 to the right of its FRT site. Exelixis, Inc. determined the insertion site of P{XP}d06654 to be at Release 3 genomic coordinate 8380767 on chromosome arm 3L.  The cytological breakpoints of Df(3L)BSC157 predicted from the transposable element insertions sites using Release 4 coordinates are 66C12;66D5.  It failed to complement Gug03928.