Subject:
Dear Dr Gramates,
I write you in place of Maurizio Gatti and  as a coauthor of the feo
paper. The fragment used for the rescue is a XhoI genomic fragment: it
comprises  1413bp upstream the 5'transcription start site and 6bp
downstream the transcription stop site. To better clarify the
situation I attach the sequence.
If you have any other question do not hesitate to contact me again.
Sincerely
Patrizia Somma
Maria Patrizia Somma, PhD
Istituto Biologia e Patologie
Molecolari del CNR
c/o Dip. Genetica e Biologia
Molecolare
Piazzale Aldo Moro, 5
00185 Roma
> Subject: 	Question about  feo rescue construct
> Date: 	Tue, 22 Aug 2006  17:02:24  -0400	
> Dear Dr Gatti,
> I am a curator for FlyBase, and am curating the precise genomic
> coordinates of rescue constructions.  I have a question about one used
> in a paper from your lab, Verni, et al., 2004 (Current Biology
>  14:15569-1575 ).  The rescue construct in this paper is described as "a
> transgene including the entire CG11207 (feo) coding region", while the
> supplementary material seems to imply that the transgene consists of
> only the coding region.  Would it be possible for you to tell me a bit
> more about the genomic fragment comprising this rescue construct, in
> particular, does it extend past the protein coding sequence of feo,
and
> if so, what is its extent?
> Sincerely,
> Sian Gramates, FlyBase