Open Close
Christensen, S., Cook, K. (2006.12.5). Isolation and characterization of Df(2L)BSC239. 
FlyBase ID
Publication Type
Personal communication to FlyBase
PubMed ID
PubMed Central ID
Text of Personal Communication
Date: Tue, 05 Dec 2006  14:55:29  -0500
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(2L)BSC239
Cc: Jill Gresens <jgresensXXXX>, Stacey Christensen <sjchristXXXX>, mdealXXXX, kaufmanXXXX
Isolation and characterization of Df(2L)BSC239
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC239 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}CG9162[d03190] and PBac{WH}CG31640[f05536]. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of P{hsFLP}1, y[1] w[1118]; 
P{XP}CG9162[d03190]/PBac{WH}CG31640[f05536] males crossed to w[1118]; 
P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as 
larvae as described in Parks et al., Nature Genetics 36: 288-292, 
2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC239 from the segment of 
P{XP}CG9162[d03190] to the left of its FRT site and the segment of 
PBac{WH}CG31640[f05536] to the right of its FRT site. Its presence 
was verified using the PCR methods and primers described in Parks et 
al. The cytological breakpoints of Df(2L)BSC239 predicted from the 
Release 4 genomic coordinates of the transposable element insertions 
sites are 26B4;26B11. It failed to complement Kr-h1[10642] and Df(2L)ED384.
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology 
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX 
Associated Information
Associated Files
Other Information
Secondary IDs
    Language of Publication
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Data From Reference
    Aberrations (2)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)