Isolation and characterization of Df(2L)BSC239
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC239 was isolated as a FLP recombinase-induced recombination event involving P{XP}CG9162d03190 and PBac{WH}CG31640f05536. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}CG9162d03190/PBac{WH}CG31640f05536 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC239 from the segment of P{XP}CG9162d03190 to the left of its FRT site and the segment of PBac{WH}CG31640f05536 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC239 predicted from the Release 4 genomic coordinates of the transposable element insertions sites are 26B4;26B11. It failed to complement Kr-h110642 and Df(2L)ED384.