Isolation and characterization of Df(3L)BSC224
Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC224 was isolated as a FLP recombinase-induced recombination event involving P{XP}sgld09725 and PBac{WH}cornf06151. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}sgld09725/PBac{WH}cornf06151 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC224 from the segment of P{XP}sgld09725 to the left of its FRT site and the segment of PBac{WH}cornf06151 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC224 predicted from the Release 4 genomic coordinates of the transposable element insertions sites using are 65D5;65E6. Df(3L)BSC224 failed to complement binI1.