Isolation and characterization of Df(3L)BSC247
Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC247 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f03039 and P{XP}CG17090d07497. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6B females crossed to P{hsFLP}1, y1 w1118; PBac{WH}f03039/P{XP}CG17090d07497 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC247 from the segment of PBac{WH}f03039 to the left of its FRT site and the segment of P{XP}CG17090d07497 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC246 predicted from the Release 4 genomic coordinates of the transposable element insertions sites are 61C1;61C3. Df(3L)BSC247 failed to complement trh10512.