FlyBase Allele Report: Dmel\trh[10512]

(Schmidt et al., 2011, Mortimer and Moberg, 2007, Tajiri et al., 2007, Spradling et al., 1999, Isaac and Andrew, 1996, Kuo et al., 1996, Wilk et al., 1996, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage

(Harris and Whitington, 2001)

lethal | recessive

(Spradling et al., 1999, Wilk et al., 1996)

lethal - all die during embryonic stage | recessive

(Perrimon et al., 1996)

partially lethal - majority die | recessive

(Berkeley Drosophila Genome Project, 1993.6.1)

some die during embryonic stage | recessive

(Perrimon et al., 1996)

Phenotype Manifest In

abdominal lateral pentascolopidial chordotonal organ lch5 & growth cone

(Harris and Whitington, 2001)

embryonic/first instar larval cuticle

(Perrimon et al., 1996)

embryonic/larval salivary gland | embryonic stage

(Isaac and Andrew, 1996)

embryonic/larval tracheal section

(Schmidt et al., 2011, Perrimon et al., 1996)

filzkorper

(Isaac and Andrew, 1996)

pulvillus | somatic clone

(Tajiri et al., 2007)

tarsal segment 4 | somatic clone - Minute background

(Tajiri et al., 2007)

tarsal segment 5 | somatic clone - Minute background

(Tajiri et al., 2007)

tracheal branch primordium

(Wilk et al., 1996)

tracheal pit

(Isaac and Andrew, 1996)

tracheal section

(Isaac and Andrew, 1996)

unguis | somatic clone

(Tajiri et al., 2007)

unguis | somatic clone - Minute background

(Tajiri et al., 2007)

Detailed Description

Statement

Reference

Trachea are absent in homozygous trh¹⁰⁵¹² embryos, yet peripheral glial migration phenotype is normal.

(Schmidt et al., 2011)

A trh¹⁰⁵¹² heterozygous background dominantly suppresses the tracheal phenotypes found in ago¹/ago³ mutants.

(Mortimer and Moberg, 2007)

trh¹⁰⁵¹² mutant clones in the distal leg are frequently associated with morphological defects in the pretarsus, such as loss of claws and malformation of the pulvilli. When large trh¹⁰⁵¹² mutant clones are generated in a Minute background loss of claws and partial ta4/ta5 fusion is seen. Clones in more proximal segments of the leg show no discernible abnormalities. No morphological defects are observed in trh¹⁰⁵¹² mutant antenna.

(Tajiri et al., 2007)

One third of the lateral chordotonal (lch5) neuron clusters fail to project normally beyond the TP1 choice point in trh¹⁰⁵¹² homozygous mutant embryos. In these segments the growth cones fail to leave the region of the lch5 cluster or they extend ventrally but along an aberrant route. Occasionally, the lch5 axons fasciculate with the axon of v'ch1 and contribute to the segmental nerve (SN). In most hemisegments of trh¹⁰⁵¹² mutant embryos, the lch5 axon bundle does reach the intersegmental nerve (ISN) and the morphology of the fascicle appears normal in most cases.

(Harris and Whitington, 2001)

Salivary glands form closed sacs that are not connected to the foregut. The filzkorper do not elongate and tracheal pits do not form. The oesophagus, gastric caecae and Malpighian tubules are unaffected.

(Isaac and Andrew, 1996)

Germline clones produce eggs with patterning defects: trachea are missing.

(Perrimon et al., 1996)

Tracheal structures are not formed in the embryo. Phenotype is more severe than for trh¹.

(Wilk et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

trh¹⁰⁵¹²/trh[+] is a suppressor of larval tracheal system phenotype of ago³/ago¹

(Mortimer and Moberg, 2007)

trh¹⁰⁵¹²/trh[+] is a suppressor of embryonic/larval tracheal dorsal trunk phenotype of ago³/ago¹

(Mortimer and Moberg, 2007)

trh¹⁰⁵¹²/trh[+] is a suppressor of tracheal lumen phenotype of ago³/ago¹

(Mortimer and Moberg, 2007)

trh¹⁰⁵¹²/trh[+] is a suppressor of embryonic/larval tracheal lateral trunk phenotype of ago³/ago¹

(Mortimer and Moberg, 2007)

trh¹⁰⁵¹²/trh[+] is a suppressor of embryonic/larval dorsal tracheal branch phenotype of ago³/ago¹

(Mortimer and Moberg, 2007)

trh¹⁰⁵¹²/trh[+] is a suppressor of tracheal primordium phenotype of ago³/ago¹

(Mortimer and Moberg, 2007)

trh¹⁰⁵¹²/trh[+] is a suppressor of embryonic/larval ganglionic tracheal branch phenotype of ago³/ago¹

(Mortimer and Moberg, 2007)

trh¹⁰⁵¹²/trh[+] is a suppressor of tracheal fusion cell phenotype of ago³/ago¹

(Mortimer and Moberg, 2007)

NOT Suppressor of

Statement

Reference

trh¹⁰⁵¹²/trh[+] is a non-suppressor of embryonic/larval tracheal section phenotype of Scer\GAL4^btl.PS, Vhl^RNAi.UAS.cMa

(Mortimer and Moberg, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

trh¹⁰⁵¹²/+ does not lead to a significant reduction in the penetrance of Scer\GAL4^btl.PS Vhl^{dsRNA.Scer\UAS} tracheal phenotypes.

(Mortimer and Moberg, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11747

P{PZ}trh¹⁰⁵¹² ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: l(3)05967^00315a. Complements: l(3)06240⁰⁶²⁴⁰. Complements: Prm¹⁰⁶³¹. Complements: l(3)neo1¹.

(BDGP Project Members, 1994-1999)

Reversion studies prove that the P{PZ} insertion is the cause of the trh¹⁰⁵¹² mutant phenotype. trh alleles fall into an allelic series. Two lethal lines were derived that complement trh mutants, along with wild type revertants and new trh alleles. From strongest to weakest: trh¹ = trh⁸ > trh⁷ > trh¹⁰⁵¹² > trh⁶ > trh⁵ > trh⁴.

(Isaac and Andrew, 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

P1747

(Jin et al., 2001)

l(3)10512

(Zelzer and Shilo, 2000, Spradling et al., 1999, Zelzer et al., 1997, Wilk et al., 1996)

l(3)10512¹⁰⁵¹²

trachealess¹⁰⁵¹²

(Okegbe and DiNardo, 2011)

trh¹⁰⁵¹²

(Schmidt et al., 2011, Christensen et al., 2009.5.6, Cook et al., 2009.11.25, Mortimer and Moberg, 2009, Araujo et al., 2007, Christensen et al., 2007.10.29, Tajiri et al., 2007, Gresens and Cook, 2006.12.5)

trh³

(Isaac and Andrew, 1996)

trh^P1747

(Jin et al., 2001)

Name Synonyms

Secondary FlyBase IDs

References (24)

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