FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\VhlRNAi.UAS.cMa
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General Information
Symbol
Dmel\VhlRNAi.UAS.cMa
Species
D. melanogaster
Name
FlyBase ID
FBal0282768
Feature type
allele
Associated gene
Dmel\Vhl
Associated Insertion(s)
Carried in Construct
P{UAS-Vhl.RNAi.M}
Key Links
Transgenic product class
RNAi_reagent
(Mortimer and Moberg, 2009)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Mortimer and Moberg, 2009)
Mutagen
in vitro construct
(Mortimer and Moberg, 2009)
Progenitor genotype
Carried in construct
P{UAS-Vhl.RNAi.M}
Cytology
Description

UAS drives expression of a Vhl RNAi construct.

(Mortimer and Moberg, 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Vhl
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of VhldsRNA.Scer\UAS.cMa under the control of Scer\GAL4tey-5053A results in an increase in the number of tracheal branches that terminate on ventrolateral body wall muscle 12 compared to controls.

      (Mortimer and Moberg, 2013)

      Scer\GAL4btl.PS-mediated expression of strongly expressing VhldsRNA.Scer\UAS lines produces migration defects in the dorsal trunk and dorsal/ventral branch, and interruptions in the lateral trunk. Expression of milder lines leads o fewer migration and fusion defects per embryo and sinuous overgrowth of the primary and secondary branches. Knockdown also produces overgrown and intertwined secondary branches and secondary branch duplication.

      During the third instar, Scer\GAL4btl.PS VhldsRNA.Scer\UAS larvae show increases in thick terminal branches and duplication of larval tertiary branches, and failure of lateral trunk fusion associated with cells terminating in multiple fine extensions.

      Most Scer\GAL4btl.PS VhldsRNA.Scer\UAS animals develop through larval stages and die as pupae.

      Scer\GAL4Act.PU-mediated expression of VhldsRNA.Scer\UAS leads to the appearance of ectopic tracheal placodes at the tp0 position and, at lower frequency, at the tp1 and tp11 positions.

      (Mortimer and Moberg, 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      Scer\GAL4btl.PS, VhlRNAi.UAS.cMa has lethal phenotype, suppressible by sima[+]/simaKG07607

      (Mortimer and Moberg, 2009)

      Scer\GAL4btl.PS, VhlRNAi.UAS.cMa has lethal phenotype, suppressible by Df(3R)BSC502/+

      (Mortimer and Moberg, 2009)
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      Scer\GAL4btl.PS, VhlRNAi.UAS.cMa has embryonic/larval tracheal section phenotype, non-suppressible by trh10512/trh[+]

      (Mortimer and Moberg, 2009)
      Enhancer of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of agoΔF.Scer\UAS and VhldsRNA.Scer\UAS.cMa under the control of Scer\GAL4tey-5053A results in a synergistic increase in the number of tracheal branches that terminate on ventrolateral body wall muscle (VLM) 12 compared to larvae expressing either agoΔF.Scer\UAS or VhldsRNA.Scer\UAS.cMa alone under the control of Scer\GAL4tey-5053A. In addition to increasing the branching of the LF and LH terminal cells onto VLM12 compared to each single mutant, the double mutants show recruitment of ectopic branches from the LG lateral terminal cell onto VLM12 in approximately 10% of cases.

      Co-expression of agoScer\UAS.cMa partially suppresses the phenotype of an increased number of tracheal branches terminating on VLM12 caused by expression of VhldsRNA.Scer\UAS.cMa under the control of Scer\GAL4tey-5053A.

      (Mortimer and Moberg, 2013)

      simaKG07607/+ significantly reduces the penetrance of Scer\GAL4btl.PS VhldsRNA.Scer\UAS tracheal defects, while simaKG07607/simaKG07607 further suppresses these defects to background levels.

      simaKG07607/+ completely suppresses the pupal lethality of Scer\GAL4btl.PS VhldsRNA.Scer\UAS.

      btldev1/+ or bnl00857/+, but not trh10512/+, leads to a significant reduction in the penetrance of Scer\GAL4btl.PS VhldsRNA.Scer\UAS tracheal phenotypes.

      ago3/+ enhances the tracheal phenotypes seen when Scer\GAL4btl.PS mediates expression of relatively weaker VhldsRNA.Scer\UAS lines, particularly enhancing the penetrance of migration defects.

      (Mortimer and Moberg, 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      VhlRNAi.UAS.cMa
      VhldsRNA.Scer\UAS.cMa
      VhldsRNA.UAS.cMa
      Name Synonyms
      Secondary FlyBase IDs
        References (3)