Isolation and characterization of Df(2R)BSC199
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC199 was isolated as a FLP recombinase-induced recombination event involving P{XP}d10427 and PBac{RB}Odae03814. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d10427/PBac{RB}Odae03814 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC199 from the segment of P{XP}d10427 to the left of its FRT site and the segment of PBac{RB}Odae03814 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. The cytological breakpoints of Df(2R)BSC199 predicted from the transposable element insertions sites using Release 4 coordinates are 48D1;48E4. It failed to complement jebk05644.