Isolation and characterization of Df(3R)BSC248
Stacey Christensen, Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC248 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}DppIIIf00363and P{XP}CG8036d01075. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6B, Tb1 females crossed to P{hsFLP}1, y1 w1118; Bac{WH}DppIIIf00363/P{XP}CG8036d01075 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC248 from the segment of Bac{WH}DppIIIf00363 to the left of its FRT site and the segment of P{XP}CG8036d01075to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC248 predicted from the Release 4 genomic coordinates of the transposable element insertions sites are 84F13;85A5. Df(3R)BSC248 failed to complement l(3)85Aaprl41 and stckEY06672.