Isolation and characterization of Df(2R)BSC307
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC307 was isolated as a FLP recombinase-induced recombination event involving P{XP}d00459 and PBac{WH}Cp1f00385. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d00459/PBac{WH}Cp1f00385 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC307 from the segment of P{XP}d00459 to the left of its FRT site and the segment of PBac{WH}Cp1f00385 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC307 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 50B6;50C18. It failed to complement fl(2)dk16105 and shot3.