Isolation and characterization of Df(2R)BSC305
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC305 was isolated as a FLP recombinase-induced recombination event involving P{XP}d06853 and PBac{WH}fdlf02041. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d06853/PBac{WH}fdlf02041 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC305 from the segment of P{XP}d06853 to the left of its FRT site and the segment of PBac{WH}fdlf02041 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC305 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 49A4;49A10. It failed to complement LacBG01462 and Df(2R)BSC3.