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General Information
Symbol
Dmel\LacBG01462
Species
D. melanogaster
Name
FlyBase ID
FBal0147818
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Lac1
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Insertion components
P{GT1}LacBG01462
Product class / Tool use(s)
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In LacBG01462 homozygous embryos, the early events of tracheal development, such as guidance, primary branching, branch fusion and extension of terminal branches occur normally. However, from stage 15 onwards most branches become more sinuous or convoluted than in the wild-type; this is especially conspicuous in the dorsal trunk. The convoluted shape of the tracheal tubes is a consequence of having lengthened too much. However, this extra growth does not appear to derive from an increase in the number of embryonic tracheocytes, but rather from an increase in the length of the cells. Tracheal lumens in these embryos are uneven, particularly those of the dorsal and lateral branches, with expansions, constrictions and numerous lumenal breaks and discontinuities. Many lumenal breaks are the result from detachment of tracheal branches from one another. Cell detachments that break the tracheal tubes are found particularly in those branches where cells elongate more. Tracheal tubes in these embryos do not inflate at the end of embryogenesis. Trachea in late stage LacBG01462 homozygous embryos retain normal epithelial polarity, and normal polarization of the cytoskeleton. However, dye permeability assays show that these embryos are unable to establish or maintain the tracheal diffusion barrier. In these assays, dye is also internalized in the salivary glands, a phenotype not seen in wild-type. At late embryonic stages these embryos have necrotic tissue in the salivary gland region.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference
Phenotype Manifest In
Enhancer of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Not rescued by
Fails to rescue
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

lethality of LacBG01462 can be reverted by precise excision.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (10)