Isolation and characterization of Df(2R)BSC859
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC859 was isolated as a FLP recombinase-induced recombination event involving P{XP}SIP2d09417 and PBac{WH}fdlf02041. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}SIP2d09417/PBac{WH}fdlf02041 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC859 from the segment of P{XP}SIP2d09417 to the left of its FRT site and the segment of PBac{WH}fdlf02041 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(2R)BSC859 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  2R:8193999 ;8392638 and the cytological breakpoints predicted from these coordinates are 48F6;49A10. Df(2R)BSC859 failed to complement LacBG01462.