Isolation and characterization of Df(2L)BSC191
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC191 was isolated as a FLP recombinase-induced recombination event involving P{XP}Slobd07006 and PBac{WH}CG7221f04545. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}Slobd07006/PBac{WH}CG722f04545 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC191 from the segment of PBac{WH}CG722f04545 to the left of its FRT site and the segment of P{XP}Slobd07006 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}Slobd07006 to be at Release 3 genomic coordinate 7667292 on chromosome arm 2L, a site predicted to be within 28C1 on both the Release 3 and Release 4 genomic maps. The predicted position of PBac{WH}CG7221f04545 on the Release 4 map is 28D3.  Consequently, the predicted cytological breakpoints of Df(2L)BSC191 are 28C1;28D3. It failed to complement mtsXE-2258 and Df(2L)BSC142.