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Christensen, S., Cook, K. (2006.8.29). Isolation and characterization of Df(2L)BSC191. 
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FBrf0199313
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Date: Tue, 29 Aug 2006  15:36:09  -0400
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(2L)BSC191
Isolation and characterization of Df(2L)BSC191
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC191 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}Slob[d07006] and PBac{WH}CG7221[f04545]. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of P{hsFLP}1, y[1] w[1118]; 
P{XP}Slob[d07006]/PBac{WH}CG722[f04545] males crossed to w[1118]; 
P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as 
larvae as described in Parks et al., Nature Genetics 36: 288-292, 
2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC191 from the segment of 
PBac{WH}CG722[f04545] to the left of its FRT site and the segment of 
P{XP}Slob[d07006] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
Exelixis, Inc. determined the insertion site of P{XP}Slob[d07006] to 
be at Release 3 genomic coordinate 7667292 on chromosome arm 2L, a 
site predicted to be within 28C1 on both the Release 3 and Release 4 
genomic maps. The predicted position of PBac{WH}CG7221[f04545] on the 
Release 4 map is 28D3.  Consequently, the predicted cytological 
breakpoints of Df(2L)BSC191 are 28C1;28D3. It failed to complement 
mts[XE-2258] and Df(2L)BSC142.
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX 
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    English
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    Aberrations (2)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)