Isolation and characterization of Df(3R)BSC197
Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC197 was isolated as a FLP recombinase-induced recombination event involving P{XP}beld01555 and PBac{WH}CG9793f03361. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6B, Tb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}beld01555/PBac{WH} CG9793f03361 males. The males were heat shocked as larvae as described in Parks et al. Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC197 from the segment of P{XP}beld01555 to the left of its FRT site and the segment of PBac{WH}f03361 to the right of its FRT site. The cytological breakpoints of Df(3R)BSC197 predicted from the transposable element insertions sites using Release 4 coordinates are 85A5;85A9. It failed to complement hb12.