Isolation and characterization of Df(2L)BSC325
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC325 was isolated as a FLP recombinase-induced recombination event involving P{XP}d03995 and PBac{WH}CG15133f05507. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d03995/PBac{WH}CG15133f05507 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC325 from the segment of P{XP}d03995 to the left of its FRT site and the segment of PBac{WH}CG15133f05507 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d03995 to be at Release 3 genomic coordinate 16694253 on chromosome arm 2L. The Gene Disruption project determined the insertion site of P{XP}d03995 to be at Release 3 genomic coordinate 16694619 on arm 2L. This corresponds to 36A11 on both the Release 3 and 5 genome maps. The predicted position of PBac{WH}CG15133f05507 on the Release 5 map is 36B3. Consequently, the cytological breakpoints of Df(2L)BSC325 are predicted to be 36A11;36B3. It failed to complement gluk08819 and Df(2L)Exel6039.