Isolation and characterization of Df(3L)BSC369
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC369 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f04973a and P{XP}CG14998d02685. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}f04973a/P{XP}CG14998d02685 males. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC369 from the segment of PBac{WH}f04973a to the left of its FRT site and the segment of P{XP}CG14998d02685 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC369 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 64A1;64A7. Df(3L)BSC369 failed to complement witA12 and FaaA9.