Isolation and characterization of Df(2R)BSC339
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC339 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}mRpS28f03356 and P{XP}d04157. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}mRpS28f03356/P{XP}d04157 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC339 from the segment of PBac{WH}mRpS28f03356 to the left of its FRT site and the segment of P{XP}d04157 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC339 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 55E2;55F6. It failed to complement Df(2R)Exel7157 and Df(2R)Exel7158. Df(2R)FDD-0315676 is a synonym for Df(2R)BSC339.