Isolation and characterization of Df(3L)BSC362
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC362 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f03039 and P{XP}d07196. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}f03039/P{XP}d07196 males. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC362 from the segment of PBac{WH}f03039 to the left of its FRT site and the segment of P{XP}d07196 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d07196 to be at Release 3 genomic coordinate 608800 on chromosome arm 3L. This corresponds to 61C7 on both the Release 3 and 5 genome maps. The predicted position of PBac{WH}f03039 on the Release 5 map is 41C1. Consequently, the cytological breakpoints of Df(3L)BSC362 are predicted to be 61C1;61C7. Df(3L)BSC362 failed to complement trh10512 and Df(3L)BSC247. Df(3L)FDD-0308240 is a synonym for Df(3L)BSC362.