Isolation and characterization of Df(3L)BSC363
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC363 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}bab1f02681 and P{XP}d01193. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}bab1f02681/P{XP}d01193 males. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC363 from the segment of PBac{WH}bab1f02681 to the left of its FRT site and the segment of P{XP}d01193 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d01193 to be at Release 3 genomic coordinate 1315105 on chromosome arm 3L. The Gene Disruption project determined the insertion site of P{XP}d01193 to be at Release 3 genomic coordinate 1315208 on arm 3L. This corresponds to 61F6 on both the Release 3 and 5 genome maps. The predicted position of PBac{WH}bab1f02681 on the Release 5 map is 61E3. Consequently, the cytological breakpoints of Df(3L)BSC363 are predicted to be 61E3;61F6. Df(3L)BSC363 failed to complement Sac1BG02228 and Klp61F07012. Df(3L)FDD-0299335 is a synonym for Df(3L)BSC363.