Isolation and characterization of Df(3L)BSC367
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC367 was isolated as a FLP recombinase-induced recombination event involving P{XP}Sk2d05405 and PBac{WH}CG14962f03417. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}Sk2d05405/PBac{WH}CG14962f03417 males. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC367 from the segment of P{XP}Sk2d05405to the left of its FRT site and the segment of PBac{WH}CG14962f03417 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC367 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 63A3;63B8. Df(3L)BSC367 failed to complement raspm47. Df(3L)FDD-0069207 is a synonym for Df(3L)BSC367.