Isolation and characterization of Df(3L)BSC381
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC381 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG10638f01666 and P{XP}CG14118d06432. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}CG10638f01666/P{XP}CG14118d06432 males. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC381 from the segment of PBac{WH}CG10638f01666  to the left of its FRT site and the segment of P{XP}CG14118d06432 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC381 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 69C4;69E8. Df(3L)BSC381 failed to complement Ptp69D1.