Open Close
Reference
Citation
Kato, M., Kato, Y., Nishida, M., Hayakawa, T., Haraguchi, T., Hiraoka, Y., Inoue, Y.H., Yamaguchi, M. (2007). Functional domain analysis of human HP1 isoforms in Drosophila.  Cell Struct. Funct. 32(1): 57--67.
FlyBase ID
FBrf0201574
Publication Type
Research paper
Abstract

Three subtypes of HP1, a conserved non-histone chromosomal protein enriched in heterochromatin, have been identified in humans, HP1alpha, beta and gamma. In the present study, we utilized a Drosophila system to characterize human HP1 functions. Over-expression of HP1beta in eye imaginal discs caused abnormally patterned eyes, with reduced numbers of ommatidia, and over-expression of HP1gamma in wing imaginal discs caused abnormal wings, in which L4 veins were gapped. These phenotypes were specific to the HP1 subtypes and appear to reflect suppressed gene expression. To determine the molecular domains of HP1 required for each specific phenotype, we constructed a series of chimeric molecules with HP1beta and HP1gamma. Our data show that the C-terminal chromo shadow domain (CSD) of HP1gamma is necessary for HP1gamma-type phenotype, whereas for the HP1beta-type phenotype both the chromo domain and the CSD are required. These results suggest human HP1 subtypes use different domains to suppress gene expression in Drosophila cells.

PubMed ID
PubMed Central ID
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell Struct. Funct.
    Title
    Cell Structure and Function
    Publication Year
    1975-
    ISBN/ISSN
    0386-7196
    Data From Reference