From: Kevin Cook <kcook@bio.indiana.edu>

To: flybase-updates@morgan.harvard.edu

Cc: Kim Cook <ruacookindiana.edu>, Stacey Christensen <sjchristindiana.edu>

Subject: Isolation and characterization of Df(3L)BSC417

Date: Tue, 15 Apr 2008 14:21:35 -0400 ( 19:21 BST)

Isolation and characterization of Df(3L)BSC417

Stacey Christensen, Kim Cook and Kevin Cook

Bloomington Stock Center

Indiana University

Df(3L)BSC417 was isolated as a FLP recombinase-induced recombination

event involving P{XP}d04722 and PBac{WH}f03787. The deletion was

isolated as a chromosome lacking miniwhite markers in progeny of

w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1]

w[1118]; P{XP}d04722/PBac{WH}f03787 males. The males were heat

shocked as larvae as described in Parks et al., Nature Genetics 36:

288-292, 2004 (FBrf0175003). This cross and crosses in preceding and

succeeding generations maintained the original genetic background of

the Exelixis insertion stocks (Thibault et al., Nature Genetics 36:

283-287, 2004; FBrf0175002). The recombination event generated the

genetic element P+PBac{XP5.WH5}BSC417 from the segment of P{XP}d04722

to the left of its FRT site and the segment of PBac{WH}f03787 to the

right of its FRT site. Its presence was verified using the PCR

methods and primers described in Parks et al. The cytological

breakpoints of Df(3L)BSC417 predicted from the Release 5 genomic

coordinates of the transposable element insertions sites are

76A1;76B9. Df(3L)BSC417 failed to complement ash1[B1] and Taf6[1].

__________________________________________________________

Kevin Cook, Ph.D. Bloomington Drosophila Stock Center

Department of Biology http://flystocks.bio.indiana.edu

Jordan Hall 142

Indiana University 812-856-1213

1001 E. Third St. 812-855-2577 (fax)

Bloomington, IN 47405-7005 kcook@bio.indiana.edu