Isolation and characterization of Df(2L)BSC332
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC332 was isolated as a FLP recombinase-induced recombination event involving P{XP}CG10600d07626 and PBac{WH}Nakf04720. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}CG10600d07626/PBac{WH}Nakf04720 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC332 from the segment of P{XP}CG10600d07626 to the left of its FRT site and the segment of PBac{WH}Nakf04720 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC332 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 37B1;37B7. Df(2L)BSC332 failed to complement tup1 and Df(2L)ED1203.