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General Information
Symbol
Dmel\tup1
Species
D. melanogaster
Name
FlyBase ID
FBal0017279
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
tupIIIB29, tupIIIB
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G18865629A

Reported nucleotide change:

G?A

Amino acid change:

C57Y | tup-PA; C57Y | tup-PB; C57Y | tup-PC

Reported amino acid change:

C57Y

Comment:

TGT to TAT change in codon C57.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: C57Y.

Nucleotide substitution: G?A.

The C57Y substitution is within the 'LIM' domain of the expressed tup product.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

tup1/tupex4 embryos exhibit an increased number of gonads with multiple niche aggregates when compared to controls.

Mutants do not show loss of expression of P{FMRFa-EGFP.Tv}, a marker for the six Ap4 neurons in the developing ventral nerve cord.

Many antero-dorsal antennal lobe projection neurons (adPN) in tup1 homozygous somatic clones fail to target the correct glomeruli although in some cases (antennal glomeruli D and VA1d) targeting occur correctly. Mis-targeted dendrites from these neurons frequently end up occupying glomeruli DA1. Many lateral antennal lobe projection neurons (lPN) in these clones are also mis-targeted with a fraction of the dendrites ending up in the subesophageal ganglion and others spread diffusely throughout the antennal lobe. However, targeting of DA1 and DL3 by lPN occurs normally. Targeting of ventral antennal lobe projection neurons (vPN) in these clones occurs normally.

tup1 die as embryos with a prominent dorsal hole.

In tup1 mutant embryos the dorsal trunk is continuous and secondary branching is indistinguishable from wild-type.

The posterior half of the abdomen is folded back on the anterior half, due to a failure of germband retraction, resulting in an eponymous phenotype. At full germband extension A8 is often opposite A2. Head involution fails. Some components of the head skeleton are absent or rudimentary, including the dorsal arms, dorsal bridge and vertical plate. The filzkorper are often widely separated. Anti-β-tubulin staining reveals a premature loss of amnioserosa cells after stage 11. The amnioserosa does not undergo programmed cell death.

Homozygous embryos exhibit an intermediate germband retraction defective phenotype. Kr staining reveals abnormalities in the amnioserosa.

Germband retraction and dorsal closure fail to occur in homozygous embryos.

Nondefective in gonad assembly.

Does not interact with RpII140wimp maternal effect.

Suppression of tor13D embryos: increase in proportion of embryos that formed cuticle and decrease in proportion of embryos that formed empty sacs.

apparently ceases early, resulting in the posteriormost three segments remaining on dorsal side of the embryo. embryonic lethal. Head broad. Germband shortening

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference

tup1 has phenotype, enhanceable by ush2

Enhancer of
Statement
Reference

tup1/tup[+] is an enhancer of thorax phenotype of ChiE

tup1 is an enhancer of phenotype of ush2

Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

tup1 enhances the excess scutellar bristle and thoracic cleft phenotype of ChiE flies when heterozygous.

Germ band retraction defects are more severe than the single homozygote when in combination with ush2.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
Comments
Comments

Both midgut primordia extend normally along the visceral mesoderm and the yolk is completely engulfed by the endoderm.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (28)